منابع مشابه
Secretion of cell-wall glycoproteins by yeast protoplasts. Effect of 2-deoxy-D-glucose and cycloheximide.
The effect of 2-deoxy-d-glucose and cycloheximide on the synthesis and secretion of the cell-wall constituents protein and mannan in yeast protoplasts was examined in detail. Although the 2-deoxy-d-glucose hardly influenced protein synthesis, a significant parallel inhibition of carbohydrate and protein secretion into the medium was observed. The mechanism of this inhibition is considered as an...
متن کاملPhysical and chemical properties of polysaccharides and glycoproteins of the yeast-cell wall.
Shockman, G. D. & Toennies, G. (1954). Arch. Biochem. Biophys. 50, 9. Stadtman, E. R., Novelli, G. D. & Lipmann, F. (1951). J. biol. Chem. 191, 365. Stekol, J. A. (1955). In Amino Acid Metabolism, p. 509. Ed. by McElroy, W. D. & Glass, H. B. Baltimore: Johns Hopkins Press. Stickland, L. H. (1951). J. gen. Microbiol. 5, 698. Szulmajster, J. & Woods, D. D. (1955). Abstr. 3rd int. Congr. Biochem.,...
متن کاملExtraction of two active polysaccharides from the yeast cell wall.
Yeast cell wall matrix particles are composed entirely of beta-glucan and mannoprotein. Alkali-insoluble (1-->3)-beta-D-glucan was extracted from the yeast cell wall by an alkaline-acid method. IR spectra analysis showed that the product was chemically pure glucan, that is to say, it contained no other carbohydrates and proteins. So, the alkaline-acid method was ideal for extracting (1-->3)-bet...
متن کاملCell wall regeneration in Chlamydomonas: accumulation of mRNAs encoding cell wall hydroxyproline-rich glycoproteins.
The unicellular alga Chlamydomonas reinhardtii is surrounded by a cell wall composed entirely of hydroxyproline-rich glycoproteins (HRGPs). When the walls of vegetative cells are removed with the enzyme gamete autolysin (g-lysin), they regenerate a matrix within 3-4 hr. In vitro translation of mRNAs isolated from g-lysin-treated cells showed significant increases and decreases in abundance of s...
متن کاملThe Structure of the Yeast Cell Wall
The autolytic release of fl-fructofuranosidase (EC 3.2.1.26) and acid phosphatase (EC 3.1.3.2) from the cell wall of yeast was studied under different organic solvents and with a variety of pII and temperature conditions. /3Fructofuranoeidase (but not acid phosphatase) was found to be a convenient marker for studying changes in the wall structure. The ethyl acetate-treated cell was characterize...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1170044pa